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Brewer's spent grain and corn steep liquor as alternative culture medium substrates for proteinase production by Streptomyces malaysiensis AMT-3 BJM
Nascimento,Rodrigo Pires do; Alves Junior,Nelson; Coelho,Rosalie Reed Rodrigues.
Brewer's spent grain and corn steep liquor or yeast extract were used as the sole organic forms for proteinase production by Streptomyces malaysiensis in submerged fermentation. The influence of the C and N concentrations, as well as the incubation periods, were assessed. Eight proteolytic bands were detected through gelatin-gel-electrophoresis in the various extracts obtained from the different media and after different incubation periods, with apparent molecular masses of 20, 35, 43, 50, 70, 100, 116 and 212 kDa. The results obtained suggest an opportunity for exploring this alternative strategy for proteinases production by actinomycetes, using BSG and CSL as economically feasible substrates.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Streptomyces malaysiensis; Brewer's spent grains; Corn steep liquor; Proteinases; Submerged fermentation.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000400020
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Culturable fungal diversity of shrimp Litopenaeus vannamei boone from breeding farms in Brazil BJM
Silva,Lidiane Roberta Cruz da; Souza,Odacy Camilo de; Fernandes,Maria José dos Santos; Lima,Débora Maria Massa; Coelho,Rosalie Reed Rodrigues; Souza-Motta,Cristina Maria.
Litopenaeus vannamei, which is the most common shrimp species cultivated in the northeast of Brazil, is very susceptible to microbial diseases, and this consequently affects productivity. There are reports of bacteria, viruses and protozoa in these shrimp, but not fungi. This study aims to isolate and identify fungi present in shrimp Litopenaeus vannamei, and in their nursery waters, at two breeding farms in Brazil. The pathogenic potential of the isolates was assessed through the qualitative detection of proteases and aflatoxin B production. The 146 isolated fungi comprised 46 species. Aspergillus, Penicillium and Furarium were the three most relevant genera and Aspergillus flavus was the predominant species with a total of 33 isolates. Most of the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Shrimp; Fungi; Water; Protease; Aflatoxin B.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000100007
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Streptomyces lunalinharesii 235 prevents the formation of a sulfate-reducing bacterial biofilm BJM
Rosa,Juliana Pacheco da; Tibúrcio,Samyra Raquel Gonçalves; Marques,Joana Montezano; Seldin,Lucy; Coelho,Rosalie Reed Rodrigues.
ABSTRACT Streptomyces lunalinharesii strain 235 produces an antimicrobial substance that is active against sulfate reducing bacteria, the major bacterial group responsible for biofilm formation and biocorrosion in petroleum reservoirs. The use of this antimicrobial substance for sulfate reducing bacteria control is therefore a promising alternative to chemical biocides. In this study the antimicrobial substance did not interfere with the biofilm stability, but the sulfate reducing bacteria biofilm formation was six-fold smaller in carbon steel coupons treated with the antimicrobial substance when compared to the untreated control. A reduction in the most probable number counts of planktonic cells of sulfate reducing bacteria was observed after treatments...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Streptomyces; Antimicrobial substance; Biofilm; Biocorrosion; Sulfate reducing bacteria.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000300603
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